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human wild type klf4  (Addgene inc)


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    Structured Review

    Addgene inc human wild type klf4
    Human Wild Type Klf4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmxs+klf4/pMXs-hKLF4+(Plasmid+%2317219)/us12559730-444-30-34
    Average 93 stars, based on 110 article reviews
    human wild type klf4 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: TET3 protects the Dlk1-Dio3 imprinted locus from DNA hypomethylation during adult NSC reprogramming
    Article Snippet: .. To produce retroviruses expressing Oct4 and Klf4 , PlatinumE (Plat-E) retroviral packing cells (RV-101, Cell Biolabs) were transfected with a plasmid solution containing 1 mL of Opti-MEMTM (Gibco), 60 μL of 1mg mL -1 polyethylenimine (PEI, Polysciences) and 20 μg of the retroviral vectors pMXs- Oct4 (#13366, Addgene), pMXs- Klf4 (#13370, Addgene) and pMXs- mCherry (pMX-2A-CH, designed and kindly provided by Dr. Jose Manuel Torres). ..

    Article Title: TET3 protects the Dlk1-Dio3 Imprinted Locus from DNA hypomethylation during adult NSC Reprogramming
    Article Snippet: .. To produce retroviruses expressing Oct4 and Klf4 , Platinum-E (Plat-E) retroviral packing cells (Cell Biolabs) were transfected with a plasmid solution containing 1 mL of Opti-MEM TM (Gibco), 60 μL of 1mg mL −1 polyethylenimine (PEI, Polysciences) and 20 μg of the retroviral vectors pMXs- Oct4 (#13366, Addgene), pMXs- Klf4 (#13370, Addgene) and pMXs- mCherry (pMX-2A-CH, designed and kindly provided by Dr. Jose Manuel Torres). ..

    Retroviral:

    Article Title: TET3 protects the Dlk1-Dio3 imprinted locus from DNA hypomethylation during adult NSC reprogramming
    Article Snippet: .. To produce retroviruses expressing Oct4 and Klf4 , PlatinumE (Plat-E) retroviral packing cells (RV-101, Cell Biolabs) were transfected with a plasmid solution containing 1 mL of Opti-MEMTM (Gibco), 60 μL of 1mg mL -1 polyethylenimine (PEI, Polysciences) and 20 μg of the retroviral vectors pMXs- Oct4 (#13366, Addgene), pMXs- Klf4 (#13370, Addgene) and pMXs- mCherry (pMX-2A-CH, designed and kindly provided by Dr. Jose Manuel Torres). ..

    Article Title: TET3 protects the Dlk1-Dio3 Imprinted Locus from DNA hypomethylation during adult NSC Reprogramming
    Article Snippet: .. To produce retroviruses expressing Oct4 and Klf4 , Platinum-E (Plat-E) retroviral packing cells (Cell Biolabs) were transfected with a plasmid solution containing 1 mL of Opti-MEM TM (Gibco), 60 μL of 1mg mL −1 polyethylenimine (PEI, Polysciences) and 20 μg of the retroviral vectors pMXs- Oct4 (#13366, Addgene), pMXs- Klf4 (#13370, Addgene) and pMXs- mCherry (pMX-2A-CH, designed and kindly provided by Dr. Jose Manuel Torres). ..

    Article Title: c-Myc alone is enough to reprogram fibroblasts into functional macrophages.
    Article Snippet: MCC intermediate cells and iPSCs were cultured in the iPSC medium [Knockout DMEM, plus 20% FBS, 1% PS, mouse LIF (1000 U/ml), non-essential amino acids (NEAA, 100×), L-glutamine (100×), and β-mercaptoethanol (100×)]. iMac cells were cultured in macrophage differentiation medium [RPMI Medium 1640 basic (Gibco) supplemented with 10% FBS, 1% PS, and 100 ng/ml M-CSF (PeproTech)]. .. The retroviral constructs pMXs-GFP, pMXs-Klf4, pMXs-Sox2, pMXs-Oct4, and pMXs-c-Myc were purchased from Addgene (Cambridge, MA). .. To produce retrovirus, the indicated plasmid was transfected into Platinum-E retroviral packaging cells using Hieff Trans® Liposomal Transfection Reagent (Yeasen) as previously described [13].

    Transfection:

    Article Title: TET3 protects the Dlk1-Dio3 imprinted locus from DNA hypomethylation during adult NSC reprogramming
    Article Snippet: .. To produce retroviruses expressing Oct4 and Klf4 , PlatinumE (Plat-E) retroviral packing cells (RV-101, Cell Biolabs) were transfected with a plasmid solution containing 1 mL of Opti-MEMTM (Gibco), 60 μL of 1mg mL -1 polyethylenimine (PEI, Polysciences) and 20 μg of the retroviral vectors pMXs- Oct4 (#13366, Addgene), pMXs- Klf4 (#13370, Addgene) and pMXs- mCherry (pMX-2A-CH, designed and kindly provided by Dr. Jose Manuel Torres). ..

    Article Title: TET3 protects the Dlk1-Dio3 Imprinted Locus from DNA hypomethylation during adult NSC Reprogramming
    Article Snippet: .. To produce retroviruses expressing Oct4 and Klf4 , Platinum-E (Plat-E) retroviral packing cells (Cell Biolabs) were transfected with a plasmid solution containing 1 mL of Opti-MEM TM (Gibco), 60 μL of 1mg mL −1 polyethylenimine (PEI, Polysciences) and 20 μg of the retroviral vectors pMXs- Oct4 (#13366, Addgene), pMXs- Klf4 (#13370, Addgene) and pMXs- mCherry (pMX-2A-CH, designed and kindly provided by Dr. Jose Manuel Torres). ..

    Plasmid Preparation:

    Article Title: TET3 protects the Dlk1-Dio3 imprinted locus from DNA hypomethylation during adult NSC reprogramming
    Article Snippet: .. To produce retroviruses expressing Oct4 and Klf4 , PlatinumE (Plat-E) retroviral packing cells (RV-101, Cell Biolabs) were transfected with a plasmid solution containing 1 mL of Opti-MEMTM (Gibco), 60 μL of 1mg mL -1 polyethylenimine (PEI, Polysciences) and 20 μg of the retroviral vectors pMXs- Oct4 (#13366, Addgene), pMXs- Klf4 (#13370, Addgene) and pMXs- mCherry (pMX-2A-CH, designed and kindly provided by Dr. Jose Manuel Torres). ..

    Article Title: TET3 protects the Dlk1-Dio3 Imprinted Locus from DNA hypomethylation during adult NSC Reprogramming
    Article Snippet: .. To produce retroviruses expressing Oct4 and Klf4 , Platinum-E (Plat-E) retroviral packing cells (Cell Biolabs) were transfected with a plasmid solution containing 1 mL of Opti-MEM TM (Gibco), 60 μL of 1mg mL −1 polyethylenimine (PEI, Polysciences) and 20 μg of the retroviral vectors pMXs- Oct4 (#13366, Addgene), pMXs- Klf4 (#13370, Addgene) and pMXs- mCherry (pMX-2A-CH, designed and kindly provided by Dr. Jose Manuel Torres). ..

    Derivative Assay:

    Article Title: Modification of Lhx2 activity for ex vivo amplification of human iPSC-derived hematopoietic stem/progenitor cells
    Article Snippet: .. Mouse iPSCs were reprogrammed from embryonic fibroblasts derived from C57BL6 mice (Nihon SLC) using pMXs-Oct3/4, pMXs-Sox2, pMXs-Klf4, and pMXs-c-Myc (Plasmids #13366, #13367, #13370, and #13375, respectively; Addgene), as previously described ( ). ..

    other:

    Article Title: MyoD is essential in rhabdomyosarcoma by promoting survival through differentiation and CYLD
    Article Snippet: pMXs-Klf4 , Addgene , RRID:Addgene_17219.

    Construct:

    Article Title: c-Myc alone is enough to reprogram fibroblasts into functional macrophages.
    Article Snippet: MCC intermediate cells and iPSCs were cultured in the iPSC medium [Knockout DMEM, plus 20% FBS, 1% PS, mouse LIF (1000 U/ml), non-essential amino acids (NEAA, 100×), L-glutamine (100×), and β-mercaptoethanol (100×)]. iMac cells were cultured in macrophage differentiation medium [RPMI Medium 1640 basic (Gibco) supplemented with 10% FBS, 1% PS, and 100 ng/ml M-CSF (PeproTech)]. .. The retroviral constructs pMXs-GFP, pMXs-Klf4, pMXs-Sox2, pMXs-Oct4, and pMXs-c-Myc were purchased from Addgene (Cambridge, MA). .. To produce retrovirus, the indicated plasmid was transfected into Platinum-E retroviral packaging cells using Hieff Trans® Liposomal Transfection Reagent (Yeasen) as previously described [13].



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